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transferrin  (Thermo Fisher)


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Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
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    Structured Review

    Thermo Fisher transferrin
    Transferrin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/apo+transferrin/Transferrin+(Apo)%2C+bovine+plasma%2C+98%2B%25/pm41224179-205-14-20
    Average 94 stars, based on 1 article reviews
    transferrin - by Bioz Stars, 2026-10
    94/100 stars

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    Cell Culture:

    Article Title: ECM29/proteasome-mediated self-antigen generation by CNS-resident neuroglia promotes regulatory T cell activation.
    Article Snippet: Human oligodendrocyte progenitors were purchased from Sigma-Aldrich (Cat. #CS204496) and were cultured on Matrigel (Corning; Cat. #354230)-coated dishes with expansion media (Sigma-Aldrich; Cat. #SCM107) according to the instruction manual. .. Oli-neu Mouse Oligodendroglial Precursor Cell Line were purchased from Sigma-Aldrich (Cat.#SCC261) and were cultured on PLLcoated dishes with Oli-neu Expansion Medium: Comprised of DMEM High Glucose Medium (Cat. No. D5796) containing 10 mg/mL Insulin (Cat. No. I9278-5ML), 10 mg/mL Apo-transferrin (Cat. No. T4382), 1X B27 Supplement(50X) (ThermoFisher 17504044), 100 mM Putrescine dihydrochloride (Cat. No. P5780), 200 nM Progesterone (Cat. No. P0130), 500 nM 3,30,5-Triiodo-L-thyronine (Cat. No. T6397), 520 nM L-Thyroxin (Cat. No. T1775), and 220 nM Sodium Selenite (Cat. No. S5261). .. All solutions, includingMEMmedium (Gibco; Cat. #11090-081), ACK lysis buffer (0.15MNH4Cl, 1 mMKHCO3, 0.01M EDTA; pH 7.2– 7.4), and Phosphate-buffered saline (PBS) [137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4; pH 7.4]), were filtered through a 0.2 mm filter and kept on ice until use.

    Article Title: Transcriptomic Signatures of Monocyte-Derived Macrophages Associate With Androgen Receptor Pathway Inhibitor Progression in Prostate Cancer.
    Article Snippet: The 22Rv1 cells were cultured in RPMI1640 media with L‐Glutamine (Corning Thermo Fisher Scientific, Waltham, MA, USA), 10% FBS (GibcoTM, Thermo Fisher Scientific, Waltham, MA, USA), and 2% penicillin/streptomycin (HycloneTM, VWR, Radnor, PA, USA). .. The C42B cells were cultured in DMEM/F12 at a 4:1 ratio (GibcoTM, Thermo Fisher Scientific, Waltham, MA, USA, #11966‐025 and #11330‐032, respectively) with 10% FBS, 0.1 μg/mL insulin (GibcoTM, Thermo Fisher Scientific, Waltham, MA, USA, #12585‐014), 275 ng/mL Triiodothyronine (Sigma‐Aldrich, Millipore Sigma, Burlington, MA, USA #T5516), 88.6 ng/mL apo‐Transferrin (Sigma‐Aldrich, Millipore Sigma, Burlington, MA, USA #T1147), 4.9 ng/mL D‐Biotin (Sigma‐Aldrich, Millipore Sigma, Burlington, MA, USA #B4639), 251.8 ng/mL Adenine (Sigma‐Aldrich, Millipore Sigma, Burlington, MA, USA #A‐3159), and 2% penicillin/streptomycin. .. Patient‐derived monocytes were cultured in RPMI1640 media with L‐Glutamine, 10% FBS, 5% Glutamax (Gibco, Thermo Fisher Scientific, Waltham, MA, USA), and 2% penicillin/streptomycin.

    Article Title: Human Cerebellar Organoids with functional Purkinje Cells
    Article Snippet: .. Organoid differentiation For differentiation, feeder-free cultured human stem cells, 80% confluent, were dissociated to single cells using room temperature Accutase (Sigma) and were reaggregated in ultra-low-attachment, V-bottomed, 96-well plates (Sbio) at 6,000 cells per 100 ul per well, in a growth factor reduced chemically defined medium (gfCDM), based on the Wataya 33 , consisting of 1:1 IMDM (Gibco) and F-12 (Gibco), 5mg/ml >99% Bovine Serum Albumin (BSA) (Sigma), 1% chemically defined lipid concentrate (CDLC) (Gibco), 15ug/ml Apo-transferrin, and 450uM Mono-Thioglycerol (Sigma) with 7ug/ml insulin (Sigma), 10 μM of SB431542 (Selleckchem), 50 ng/ml Noggin (Peprotech), 1.7 μM CHIR (Reprocell), and 20 μM Y-27632 ROCK inhibitor (Stem Cell Technologies). ..

    Recombinant:

    Article Title: Factor XII signaling via uPAR-integrin β1 axis promotes tubular senescence in diabetic kidney disease
    Article Snippet: .. The Proximal tubule containing fragments were pelleted down and washed twice with HBSS, then the pellet was resuspended in DMEM:F12 medium in a ratio of 1:1, supplemented with insulin/transferrin/selenium (Invitrogen, 5 μg/ml, 2.75 μg/ml and 3.35 ng/ml respectively), APO transferrin (Sigma-Aldrich, 2.0 μg/ml), hydrocortisone (Sigma-Aldrich, 40 ng/ml), recombinant human epidermal growth factor (rhEGF, R&D Systems, 0.01 μg/ml), and 1% antibiotic/antimycotic solution (Sigma-Aldrich, 10,000 units/ml penicillin, 0.1 mg/ml streptomycin and 0.25 μg/ml amphotericin B). ..



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